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hADRB3 MAb (Cl 49332 (25 UG)
hADRB3 MAb (Cl 49332 (25 UG)
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hADRB3 MAb (Cl 49332 (25 UG)

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品牌： R&D Systems
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    来源宿主：
    Mouse IgG2b
    Mouse IgG2b
    实验应用：
    Flow Cytometry, Immunocytochemistry
    Flow Cytometry, Immunocytochemistry
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    宿主
    Mouse IgG2b
    免疫原
    Human embryonic kidney cell line HEK293-derived transfected with human beta -3 Adrenergic R/ADRB3
    Met1-Ser408
    Accession # P13945
    克隆号
    493324

    Scientific Data

    Flow Cytometry Detection of beta -3 Adrenergic R/ADRB3 antibody in HEK293 Human Cell Line Transfected with Human beta -3 Adrenergic R/ADRB3 and eGFP antibody by Flow Cytometry.View Larger

    Detection of beta ‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta ‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human beta -3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta -3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

    Immunocytochemistry beta -3 Adrenergic R/ADRB3 antibody in Human Adipocytes by Immunocytochemistry (ICC).View Larger

    beta ‑3 Adrenergic R/ADRB3 in Human Adipocytes. beta -3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta -3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

    Human beta-3 Adrenergic R/ADRB3 Antibody Summary

    Species Reactivity
    Human
    Specificity
    Stains human beta -3 Adrenergic R/ADRB3 transfectants but not irrelevent transfectants in flow cytometry.
    Source
    Monoclonal Mouse IgG2B Clone # 493324
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    Human embryonic kidney cell line HEK293-derived transfected with human beta -3 Adrenergic R/ADRB3
    Met1-Ser408
    Accession # P13945
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Flow Cytometry
    0.25 µg/106 cells
    See below
    Immunocytochemistry
    8-25 µg/mL
    See below

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Flow Cytometry, Immunocytochemistry
    背景
    别名
    ADRB3,ADRB3R,adrenergic, beta-3-, receptor,B3AR,beta-3 Adrenergic R,beta-3 adrenergic receptor,beta3 AdrenergicR,beta-3 AdrenergicR,Beta-3 adrenoceptor,Beta-3 adrenoreceptor,BETA3AR,FLJ99960,Beta-3 Adrenergic Receptor
    背景

    Background: beta-3 Adrenergic R/ADRB3

     ADRB3 belongs to the G protein-coupled receptor 1 family of beta adrenergic receptors. Beta-adrenergic receptors, including ADRB3, mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRB3 is involved in the regulation of lipolysis and thermogenesis. A variant of ADRB3 seems to be associated with weight gain (obesity).

    Long Name
    Beta-3 Adrenergic Receptor
    Entrez Gene IDs
    155 (Human); 11556 (Mouse); 25645 (Rat)
    Alternate Names
    ADRB3; ADRB3R; adrenergic, beta-3-, receptor; B3AR; beta-3 Adrenergic R; beta-3 adrenergic receptor; beta3 AdrenergicR; beta-3 AdrenergicR; Beta-3 adrenoceptor; Beta-3 adrenoreceptor; BETA3AR; FLJ99960
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    Detection of beta ‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta ‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human beta ‑3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

    beta ‑3 Adrenergic R/ADRB3 in Human Adipocytes. beta ‑3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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    货号：
    MAB9419-SP
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    询价
    25UG
    100UG
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