hc-Myc MAb (Cl 9E10) (25 ug)

Detection of Human c‑Myc by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of c‑Myc-tagged Protein by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected (-) or transfected with c-Myc-tagged recombinant mouse Wnt-3a. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc-tagged recombinant mouse Wnt-3a at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc Mono-clonal Antibody (Catalog # MAB3696, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

c-Myc in HEK293 Human Cell Line Transfected with c-Myc-tagged Serotonin Receptor. c-Myc was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with c-Myc-tagged Serotonin Receptor using Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

c‑Myc in Human Prostate. c-Myc was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human c‑Myc by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Myc at approximately 74 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human c‑Myc by Simple Western^TM. Simple Western lane view shows c-Myc-tagged recombinant human ANGPTL6, loaded at 0.2 mg/mL. A specific band was detected for c‑Myc at approximately 161 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and c-Myc knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for c-Myc at approximately 52 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.