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hIL-6Ra MAb (Cl 175 (25 ug)
hIL-6Ra MAb (Cl 175 (25 ug)
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hIL-6Ra MAb (Cl 175 (25 ug)

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品牌： R&D Systems
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    来源宿主：
    Mouse IgG1
    Mouse IgG1
    实验应用：
    Western Blot, Flow Cytometry, CyTOF-ready, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization
    Western Blot, Flow Cytometry, CyTOF-ready, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    抗原名称
    CD126， gp80， IL-6 R alpha， IL6Q， IL6R alpha， IL-6R alpha， IL6R， IL-6R-1， IL6RA， IL-6Ra， IL6RQ， inter
    宿主
    Mouse IgG1
    免疫原
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R
    Leu20-Asp339
    Accession # P08887
    克隆号
    17506

    Scientific Data

    Western Blot Detection of Recombinant Human IL-6R alpha antibody by Western Blot.View Larger

    Detection of Recombinant Human IL-6R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

    Flow Cytometry Detection of IL-6R alpha antibody in U937 Human Cell Line antibody by Flow Cytometry.View Larger

    Detection of IL-6R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was labeled with Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

    Neutralization IL-6R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody.View Larger

    IL-6R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically 0.04-0.2 µg/mL.

    Human IL-6R alpha Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human IL-6R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
    Source
    Monoclonal Mouse IgG1 Clone # 17506
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
    Leu20-Asp339
    Accession # P08887
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Western Blot
    1 µg/mL
    See below
    Flow Cytometry
    0.25 µg/106 cells
    See below
    CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
     

    Human IL-6R alpha Sandwich Immunoassay

    Recommended Concentration
    Reagent
    ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 

    Use in combination with:

    Detection Reagent: Human IL-6R alpha Biotinylated Antibody (Catalog # BAF227)

    Standard: Recombinant Human IL-6R alpha Protein (Catalog # 227-SR)

    Neutralization

    Measured by its ability to neutralize IL-6R alpha-mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL-6R alpha and 30 ng/mL Recombinant Human IL‑6.

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Western Blot, Flow Cytometry, CyTOF-ready, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization
    背景
    别名
    CD126,gp80,IL-6 R alpha,IL6Q,IL6R alpha,IL-6R alpha,IL6R,IL-6R-1,IL6RA,IL-6Ra,IL6RQ,interleukin 6 receptor,Interleukin 6 Receptor alpha
    背景

    Background: IL-6R alpha

    The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

    A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

    References
    1. Yamasaki et al. (1988) Science 241:825.
    2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
    3. Hibi et al. (1990) Cell 63:1149.
    4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
    5. Novick et al. (1989) J. Exp. Med. 170:1409.
    6. Yasukawa et al. (1990) J. Biochem. 108:673.
    7. Saito et al. (1991) J. Immunology 147:168.
    Long Name
    Interleukin 6 Receptor alpha
    Entrez Gene IDs
    3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
    Alternate Names
    CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    IL‑6 R alpha Enhancement of
    IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human
    IL‑6 R alpha Antibody.     Recombinant Human IL‑6 R alpha (Catalog #
    227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically
    0.04-0.2 µg/mL.

    Detection of IL‑6 R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was labeled with Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG
    F(ab')2 Secondary Antibody (Catalog # F0102B).

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    货号：
    MAB227-SP
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