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hIntegrin aVb3 MAb ( (25 ug)
hIntegrin aVb3 MAb ( (25 ug)

hIntegrin aVb3 MAb ( (25 ug)

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    来源宿主：
    Mouse IgG1
    Mouse IgG1
    实验应用：
    Flow Cytometry(2.5 µg/106 cells)
    Flow Cytometry(2.5 µg/106 cells)
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    抗原名称
    antigen identified by monoclonal L230， CD51 antigen， CD51， Integrin alpha V beta 3， integrin alpha-V
    宿主
    Mouse IgG1
    免疫原
    Human osteoclasts
    克隆号
    23C6

    Scientific Data

    Immunocytochemistry/ Immunofluorescence Detection of Human Integrin alpha V beta 3 by Immunocytochemistry/ImmunofluorescenceView Larger

    Detection of Human Integrin alpha V beta 3 by Immunocytochemistry/Immunofluorescence Effects of AA and DHA on actin ring formation and VNR expression.Osteoclast differentiation was stimulated in CD14+ monocytes by the addition of 25 ng ml-1 M-CSF and 30 ng ml-1 RANKL as described in Materials and Methods. Vehicle (0.08% ethanol) or LCPUFAs (40 μorption (day 12–14) in mature osteoclasts. After 3 weeks of culture, cells were fixed and stained with Hoechst (blue) for nuclei, phalloidin (red) for actin rings, and anti-VNR antibody (green). The results are representative of three independent experiments conducted in triplicate. Scale bar = 200 μm. Diff Ocl—differentiating osteoclasts. Mat Ocl—mature osteoclasts. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25867515), licensed under a CC-BY license. Not internally tested by R&D Systems.

    Human Integrin  alpha V beta 3 Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human Integrin alpha V beta 3.
    Source
    Monoclonal Mouse IgG1 Clone # 23C6
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    Human osteoclasts
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.

    Applications

    Recommended Concentration
    Sample
    Flow Cytometry
    2.5 µg/106 cells
    HUVEC human umbilical vein endothelial cells
    Immunohistochemistry
    Davies, J. et al. (1989) J. Cell Biol. 109:1817.
     
    Immunoprecipitation
    Davies, J. et al. (1989) J. Cell Biol. 109:1817.
     
    Adhesion Blockade
    Horton, M.A. et al. (1991) Exp. Cell Res. 195:368. Bates, R.C. et al. (1998) Cell Adhes. Commun. 6:21.
     
    CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
     

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Flow Cytometry(2.5 µg/106 cells)
    背景
    别名
    antigen identified by monoclonal L230,CD51 antigen,CD51,Integrin alpha V beta 3,integrin alpha-V,integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51),MSK8,Vitronectin receptor subunit alpha,VNRADKFZp686A08142
    背景

    Background: Integrin alpha V beta 3

    Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.

    References
    1. Hynes, R. O. (2002) Cell 110:673.
    2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
    3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
    4. Xiong, J. et al. (2001) Science 294:339.
    5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
    6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
    7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
    8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
    9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
    10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
    11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
    12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
    Entrez Gene IDs
    3685 (Human)
    Alternate Names
    antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
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    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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    货号：
    MAB3050-SP
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