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hIDH1 MAb (Cl 843219 (100 ug)
hIDH1 MAb (Cl 843219 (100 ug)
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hIDH1 MAb (Cl 843219 (100 ug)

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    来源宿主：
    Mouse IgG1
    Mouse IgG1
    实验应用：
    Western Blot(0.25 µg/mL)
    Immunohistochemistry(8-25 µg/mL)
    Immunocytochemistry(8-25 µg/mL)
    Western Blot(0.25 µg/mL)
    Immunohistochemistry(8-25 µg/mL)
    Immunocytochemistry(8-25 µg/mL)
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    抗原名称
    Cytosolic NADP-isocitrate dehydrogenase， EC 1.1.1.42， IDCD， IDH， IDH1， IDP， IDPC， isocitrate dehydro
    宿主
    Mouse IgG1
    免疫原
    E. coli-derived recombinant human Isocitrate Dehydrogenase 1/IDH1
    Ser2-Leu414
    Accession # O75874
    克隆号
    843219

    Scientific Data

    Western Blot Detection of Human, Mouse, and Rat Isocitrate Dehydrogenase 1/IDH1 antibody by Western Blot.View Larger

    Detection of Human, Mouse, and Rat Isocitrate Dehydrogenase 1/IDH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

    Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC).View Larger

    Isocitrate Dehydrogenase 1/IDH1 in SK‑BR‑3 Human Cell Line. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

    Immunohistochemistry Isocitrate Dehydrogenase 1/IDH1 antibody in Human Brain by Immunohistochemistry (IHC-P).View Larger

    Isocitrate Dehydrogenase 1/IDH1 in Human Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

    Immunohistochemistry Isocitrate Dehydrogenase 1/IDH1 antibody in Rat Brain by Immunohistochemistry (IHC-Fr).View Larger

    Isocitrate Dehydrogenase 1/IDH1 in Rat Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to glial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

    Knockout Validated Western Blot Shows Human Isocitrate Dehydrogenase 1/IDH1 Antibody Specificity by Using Knockout Cell Line.View Larger

    Western Blot Shows Human Isocitrate Dehydrogenase 1/IDH1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Isocitrate Dehydrogenase 1/IDH1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 46 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

    Simple Western View Larger

    Detection of Human Isocitrate Dehydrogenase 1/IDH1 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 51 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

    Human Isocitrate Dehydrogenase 1/IDH1 Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human Isocitrate Dehydrogenase 1/IDH1 in ELISAs and Western blots.
    Source
    Monoclonal Mouse IgG1 Clone # 843219
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    E. coli-derived recombinant human Isocitrate Dehydrogenase 1/IDH1
    Ser2-Leu414
    Accession # O75874
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Western Blot
    0.25 µg/mL
    See below
    Simple Western
    10 µg/mL
    HepG2 human hepatocellular carcinoma cell line
    Immunohistochemistry
    8-25 µg/mL
    See below
    Immunocytochemistry
    8-25 µg/mL
    See below
    Knockout Validated
    Isocitrate Dehydrogenase 1/IDH1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Isocitrate Dehydrogenase 1/IDH1 knockout HeLa cell line.
     

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Western Blot(0.25 µg/mL)
    Immunohistochemistry(8-25 µg/mL)
    Immunocytochemistry(8-25 µg/mL)
    背景
    别名
    Cytosolic NADP-isocitrate dehydrogenase,EC 1.1.1.42,IDCD,IDH,IDH1,IDP,IDPC,isocitrate dehydrogenase [NADP] cytoplasmic,isocitrate dehydrogenase 1 (NADP+), soluble,Isocitrate Dehydrogenase 1,NADP(+)-specific ICDH,NADP-dependent isocitrate dehydrogenase, cytosolic,NADP-dependent isocitrate dehydrogenase, peroxisomal,Oxalosuccinate decarboxylase,PICD
    背景

    Background: Isocitrate Dehydrogenase 1/IDH1

    Isocitrate Dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate to alpha ‑ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP+ as the electron acceptor and the other using NAD+ (1). The protein encoded by this gene is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. In peroxisomes, IDH1 generates the NADPH required for intraperoxisomal reduction reactions. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from  alpha ‑ketoglutarate (2). Elevated levels of the metabolite 2HG are associated with a high risk of malignant brain tumors. Arg132 mutations of IDH1 are common in high‑grade gliomas, but not in other types of tumors (3).

    References
    1. Nekrutenko, A. et al. (1998) Mol. Biol. Evol. 15:1674.
    2. Dang, L. et al. (2009) Nature 462:739.
    3. Bleeker, F.E. et al. (2009) Hum. Mutat. 30:7.
    Entrez Gene IDs
    3417 (Human); 15926 (Mouse); 24479 (Rat)
    Alternate Names
    Cytosolic NADP-isocitrate dehydrogenase; EC 1.1.1.42; IDCD; IDH; IDH1; IDP; IDPC; isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate Dehydrogenase 1; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; Oxalosuccinate decarboxylase; PICD
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    Detection of Human, Mouse, and Rat Isocitrate Dehydrogenase 1/IDH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

    Isocitrate Dehydrogenase 1/IDH1 in SK‑BR‑3 Human Cell Line. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed
    SK‑BR‑3 human breast cancer cell line using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

    Isocitrate Dehydrogenase 1/IDH1 in Human Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

    Isocitrate Dehydrogenase 1/IDH1 in Rat Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to glial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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    货号：
    MAB7049
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