hPhos PGC1 alpha Aff (100 ug)

品牌： R&D Systems

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参数

来源宿主：

Rabbit IgG

实验应用：

Western Blot(1 µg/mL)

产品介绍

产品信息

耦联标记

Unconjugated

纯化方式

Antigen Affinity-purified

抗原名称

LEM6， Ligand effect modulator 6， ligand effect modulator-6， L-PGC-1alpha， peroxisome proliferative a

宿主

Rabbit IgG

免疫原

Phosphopeptide containing the human PGC1 alpha S571 site.

Scientific Data

Western Blot

View Larger

Detection of Human Phospho-PGC1 alpha (S571) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 10 nm Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-PGC1a (S571) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6650) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PGC1a (S571) at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human Phospho-PGC1 alpha (S571) Antibody Summary

Species Reactivity

Human

Specificity

Detects human PGC1 alpha when phosphorylated at S571 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing the human PGC1 alpha S571 site.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

Western Blot(1 µg/mL)

背景

别名

LEM6,Ligand effect modulator 6,ligand effect modulator-6,L-PGC-1alpha,peroxisome proliferative activated receptor, gamma, coactivator 1, alpha,peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext,peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a,peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT,peroxisome proliferator-activated receptor gamma coactivator 1-alpha,peroxisome proliferator-activated receptor gamma, coactivator 1 alpha,PGC1 alpha,PGC1,PGC-1(alpha),PGC1A,PGC-1-alpha,PGC1APGC-1(alpha),PGC1peroxisome proliferative activated receptor, gamma, coactivator 1,PGC-1v,PPAR gamma coactivator variant form,PPAR gamma coactivator-1,PPARgamma coactivator 1alpha,PPAR-gamma coactivator 1-alpha,PPARGC1,PPARGC1A,PPARGC-1-alpha,Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

背景

Background: PGC1 alpha

PGC-1 alpha (PPAR-gamma coactivator 1; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC-1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG-1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC-1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC-1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG-1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Entrez Gene IDs

10891 (Human); 19017 (Mouse); 83516 (Rat)

Alternate Names

LEM6; Ligand effect modulator 6; ligand effect modulator-6; L-PGC-1alpha; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC1 alpha; PGC1; PGC-1(alpha); PGC1A; PGC-1-alpha; PGC1APGC-1(alpha); PGC1peroxisome proliferative activated receptor, gamma, coactivator 1; PGC-1v; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPARgamma coactivator 1alpha; PPAR-gamma coactivator 1-alpha; PPARGC1; PPARGC1A; PPARGC-1-alpha

研究领域

Related Research Areas

翻译后修饰

磷酸化

制备和贮存

溶解方法

Sterile PBS to a final concentration of 0.2 mg/mL.

保存方式

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Detection of Human Phospho-PGC1 alpha (S571) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 10 nm Recombinant Human IGF‑I (Catalog # 291-G1) for 1 hour. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-PGC1 alpha (S571) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6650) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PGC1 alpha (S571) at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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货号：

AF6650

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