Human T Cell Nucleofector® Kit

品牌： Lonza

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产品介绍

产品信息

商品描述

For efficient transfection of primary human T cells in the Nucleofector®I/II/2b System

组成成分

磷酸盐缓冲液100%、纯水100%、盐酸盐缓冲液100%

Product Overview

The Nucleofector^® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector^® Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).

The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

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Benefits

High transfection efficiencies for primary cells
Cells maintain functionality post transfection

Applications

Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cells
Low-throughput transfection in single cuvette format

Storage and Content

2.25 mL Nucleofector^® Solution for Human T Cells
0.5 mL Supplement 1
30 µg pmaxGFP^TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL), 25 Single use pipettes

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货号：

VPA-1002

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