首页
h/mNurr1 Aff Pur PAb (100 ug)
h/mNurr1 Aff Pur PAb (100 ug)
1/2

h/mNurr1 Aff Pur PAb (100 ug)

分享
品牌： R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    来源宿主：
    Goat IgG
    Goat IgG
    实验应用：
    Western Blot(0.1 µg/mL)
    Immunohistochemistry(5-15 µg/mL)
    Western Blot(0.1 µg/mL)
    Immunohistochemistry(5-15 µg/mL)
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Antigen Affinity-purified
    抗原名称
    human homolog of， NGFI-B beta， NOT， NR4A2， nuclear receptor subfamily 4, group A, member 2， Nurr1， o
    宿主
    Goat IgG
    免疫原
    E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
    Val332-Lys558
    Accession # Q06219

    Scientific Data

    Immunocytochemistry View Larger

    Nurr1/NGFI‑B beta /NR4A2 in HeLa Human Cell Line. Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

    Immunohistochemistry View Larger

    Nurr1/NGFI‑B beta /NR4A2 in Mouse Brain (Hippocampus). Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

    Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody Summary

    Species Reactivity
    Human, Mouse
    Specificity
    Detects human and mouse Nurr1/NGFI‑B beta /NR4A2 in direct ELISAs and Western blots.
    Source
    Polyclonal Goat IgG
    Purification
    Antigen Affinity-purified
    Immunogen
    E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
    Val332-Lys558
    Accession # Q06219
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Western Blot
    0.1 µg/mL
    Recombinant Mouse Nurr1/NGFI‑B beta /NR4A2
    Immunohistochemistry
    5-15 µg/mL
    Immersion fixed differentiated dopamenergic nuerons from D3 mouse embryonic stem cells
    Immunocytochemistry
    5-15 µg/mL
    Immersion fixed HeLa human cervical epithelial carcinoma cell line

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Western Blot(0.1 µg/mL)
    Immunohistochemistry(5-15 µg/mL)
    背景
    别名
    human homolog of,NGFI-B beta,NOT,NR4A2,nuclear receptor subfamily 4, group A, member 2,Nurr1,orphan nuclear receptor NR4A2,Orphan nuclear receptor NURR1,T-cell nuclear receptor NOT,TINUR,transcriptionally inducible nuclear receptor related 1,Transcriptionally-inducible nuclear receptor,Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2
    背景

    Background: Nurr1/NGFI-B beta/NR4A2

    Nurr1 belongs to the nerve growth factor-induced clone B subfamily of nuclear receptor superfamily and is designated NR4A2 in the Unified Nomenclature System for NRs. Nurr1 can function as a ligand-independent transcription factor. Nurr1 also heterodimerizes with retinoid X receptors (RXRs) to function as ligand-regulated transcription factor. Nurr1 is important for specification of dopaminergic neurons that degenerate in Parkinson’s disease. Human and mouse Nurr1 share 99% amino acid sequence homology.

    Long Name
    Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2
    Entrez Gene IDs
    4929 (Human); 18227 (Mouse)
    Alternate Names
    human homolog of; NGFI-B beta; NOT; NR4A2; nuclear receptor subfamily 4, group A, member 2; Nurr1; orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; T-cell nuclear receptor NOT; TINUR; transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.2 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    AF2156
    一键复制
    询价
    25ug
    100ug
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价