BD™ CompBeads Anti-Mouse Ig, κ/Negative Control Compensation Particles Set

The BD™ CompBeads Set Anti-Mouse Ig, κ are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD™ CompBeads Anti-Mouse Ig, κ particles, which bind any mouse κ light chain-bearing immunoglobulin, and the BD™ CompBeads Negative Control, which has no binding capacity. When mixed together with a fluorochrome-conjugated mouse antibody, the BD™ CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

(RUO)

Flow cytometry (Routinely Tested)

Ig, κ Light Chain