BD Pharmingen™ ICC Fixation Buffer

ICC Fixation Buffer is useful for fixing unstained cells that have been adhered to microscopic slides for cytokine immunocytochemical staining.  This fixation buffer is intended to preserve human and rodent lymphoid cells for the subsequent immunocytochemical staining of intracellular cytokines.  Once fixed the slides may be stored at -80°C for up to two months . When stored under those conditions no changes in cell morphology have been observed and the levels of intracellular cytokine detected by immunocytochemistry remain unaffected. Note: The suitability of fixation conditions for cells undergoing immunocytochemistry staining depends on the availability of compatible antibodies that can specifically detect their cognate antigens under the same fixation conditions. With respect to intracellular cytokines and chemokines, BD Pharmingen offers a panel of purified anti-cytokine antibodies optimized for use in immunocytochemistry of cells fixed with formalin.

(RUO)

(Routinely Tested)

ICC Fixation Buffer

Development References(10) 1. Andersson J, Abrams J, Bjork L, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994; 83(1):16-24. (Biology). 2. Andersson J, Bjork L, Dinarello CA, Towbin H, Andersson U. Lipopolysaccharide induces human interleukin-1 receptor antagonist and interleukin-1 production in the same cell. Eur J Immunol. 1992; 22(10):2617-2623. (Biology). 3. Andersson J, Fehniger TE, Patterson BK, et al. Early reduction of immune activation in lymphoid tissue following highly active HIV therapy. AIDS. 1998; 12(11):F123-F129. (Biology). 4. Barnes PF, Lu S, Abrams JS, Wang E, Yamamura M, Modlin RL. Cytokine production at the site of disease in human tuberculosis. Infect Immun. 1993; 61(8):3482-3489. (Biology). 5. Bjork L, Fehniger TE, Andersson U, Andersson J. Computerized assessment of production of multiple human cytokines at the single-cell level using image analysis. J Leukoc Biol. 1996; 59(2):287-295. (Biology). 6. Bjork L. Development of an image analysis system for the evaluation of cytokine production. Stockholm: 1995. 7. Hsu SM, Raine L, Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol. 1981; 75(5):734-738. (Biology). 8. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-580. (Biology). 9. Raqib R, Lindberg AA, Bjork L, et al. Down-regulation of gamma interferon, tumor necrosis factor type I, interleukin 1 (IL-1) type I, IL-3, IL-4, and transforming growth factor beta type I receptors at the local site during the acute phase of Shigella infection. Infect Immun. 1995; 63(8):3079-3087. (Biology). 10. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Biology).