BD™ Cytometric Bead Array (CBA) Mouse IFN-γ Flex Set

Figure 1. Example BD CBA Mouse IFN-γ Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Mouse IFN-γ Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Figure 1.  Example BD CBA Mouse IFN-γ Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Mouse IFN-γ Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.