

品牌: BD Pharmingen








反应种属:
Mouse (QC Testing)
Mouse (QC Testing)
来源宿主:
Rat IgG2a, κ
Rat IgG2a, κ
产品介绍
产品信息
耦联标记
BV510

抗原名称
IgD

宿主
Rat IgG2a, κ

免疫原
Not reported

商品描述
The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all
Igh-C
haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes. Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells
in vitro
.
In vivo
injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

同种型
Rat IgG2a, κ

克隆号
克隆 11-26c.2a (RUO)

浓度
0.2 mg/ml

应用
实验应用
Flow cytometry (Routinely Tested)

反应种属
Mouse (QC Testing)

目标/特异性
IgD

背景
别名
IGHD; Igh-5; Immunoglobulin heavy chain 5; Ig delta chain C region

制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
380797

参考图片
Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; Left Panel) or BD Horizon™ BV510 Rat anti-Mouse IgD antibody (Cat. No. 563110; Right Panel). Two-color flow cytometric dot plots showing the correlated expression patterns of IgD (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR™ II Flow Cytometer System.
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