BD Horizon™ BV786 Rat Anti-Mouse IgD

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BD Horizon™ BV786 Rat Anti-Mouse IgD

品牌： BD Pharmingen

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参数

反应种属：

Mouse (QC Testing)

来源宿主：

Rat IgG2a, κ

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产品介绍

产品信息

耦联标记

BV786

抗原名称

IgD

宿主

Rat IgG2a, κ

免疫原

Not reported

简单描述

The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all Igh-C haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes. Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells in vitro . In vivo injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.

商品描述

11-26c.2a The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all Igh-C haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes. Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells in vitro . In vivo injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.

同种型

Rat IgG2a, κ

克隆号

克隆 11-26c.2a (RUO)

浓度

0.2 mg/ml

产品详情

BV786

The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm. BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.

格式:BV786

激发源:Violet 405 nm

最多激发源:407 nm

最多发射源:786 nm

应用

实验应用

Flow cytometry (Routinely Tested)

反应种属

Mouse (QC Testing)

目标/特异性

IgD

背景

别名

IGHD; Igh-5; Immunoglobulin heavy chain 5; Ig delta chain C region

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(5) 1. BD Biosciences Pharmingen. Unpublished results. . 2. Campbell KS, Cambier JC. B lymphocyte antigen receptors (mIg) are non-covalently associated with a disulfide linked, inducibly phosphorylated glycoprotein complex. EMBO J. 1990; 9(2):441-448. (Clone-specific: Immunoprecipitation). 3. Hamilton AM, Lehuen A, Kearney JF. Immunofluorescence analysis of B-1 cell ontogeny in the mouse. Int Immunol. 1994; 6(3):355-361. (Clone-specific: Flow cytometry, Immunofluorescence). 4. Ishihara K, Wood WJ Jr, Wall R, et al. Multiple B29 containing complexes on murine B lymphocytes. Common and stage-restricted Ig-associated polypeptide chains. J Immunol. 1993; 150(6):2253-2262. (Clone-specific: Flow cytometry). 5. Nitschke L, Kosco MH, Kohler G, Lamers MC. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. Proc Natl Acad Sci U S A. 1993; 90(5):1887-1891. (Clone-specific: Flow cytometry).

数据库链接

Entrez-Gene ID

380797

参考图片

Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either BD Horizon™ BV786 Rat IgG2a, κ Isotype Control (Cat. No. 563335, Left Panel) or BD Horizon™ BV786 Rat Anti-Mouse IgD antibody (Cat. No. 563618, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of IgD (or Ig isotype control staining) and CD3e were derived from gated events with the forward and side light scattering characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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货号：

563618

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