Purified Dog IgE

Unconjugated

Monoclonal canine IgE specific for a nematode antigen was isolated from the cell culture supernatant of a canine x-mouse heterohybridoma cell line using protein A affinity chromatography.Concentration is 1. 0 mg/ml determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1. 0 mg of IgE.By SDS-PAGE nonreduced gels show only one band, at 200 kilodaltons (kd), indicating 95% purity, and reduced gels show only two bands, at 75 kd and 25 kd. This protein at concentrations as low as 1 ng/ml is detected by mouse monoclonal Antibodies with biotin, binds to canine mast cells as detected by avidin-phycoerythrin using flow cytometry.

狗

IgE monomers contain two heavy chains and two light chains, and are primarily involved in the immune response to parasites, and has been associated with allergies and asthma.

Purified

犬抗线虫抗原免疫球E型蛋白抗体，三羟甲基氨基甲烷缓冲液（含0.09%叠氮化钠溶液）