Anti-mouse IgG (H+L), F(ab‘) 2 Fragment (Alexa Fluor ® 555 Conjugate)

Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb #2317 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞，所用抗体为S6 Ribosomal Protein (54D2) Mouse mAb #2317，检测抗体为anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate) (上图)，与同型对照（下图）作比较。 肌动蛋白丝采用fluorescein phalloidi标记。蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084。

High content analysis of C2C12 cells exposed to varying concentrations of LY294002 #9901 for 2 hours. With increasing concentrations of LY294002, a significant decrease (~20 fold) in phospho-S6 Ribosomal protein (Ser235/236) signal as compared to the untreated control was observed. When using phospho-S6 Ribosomal protein as a measurement, the IC50 of this compound was 2.3 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate).高通量分析C2C12细胞，该细胞暴露于LY294002 #9901 2个小时。与未处理的对照组相比较，随着LY294002 浓度的增加phospho-S6 Ribosomal protein (Ser235/236)信号显著降低（约20倍）。当采用 phospho-S6 Ribosomal protein 检测时，IC50为2.3mM。数据时以Acumen ® HCS为平台生成的，所用抗体为Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate)。