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Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)

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Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)

品牌： CST

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参数

反应种属：

Mouse

来源宿主：

Mouse

产品介绍

产品信息

耦联标记

HRP

抗原名称

IgG

来源纯化

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

宿主

Mouse

简单描述

Secondary Antibody for studying Rabbit IgG in the research area.

商品描述

Product Usage Information

Recommended Antibody Dilution:1:2000

应用

反应种属

Mouse

目标/特异性

Specificity/Sensitivity

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. This antibody may cross react with denatured [light chain] IgG at high concentrations.

Species Reactivity:

Rabbit

背景

Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.

制备和贮存

保存方式

Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

参考图片

Immunoprecipitation of PRAS40 from HeLa cells using PRAS40 (D23C7) XP® Rabbit mAb #2691 (lane 2). Lane 1 contains input control lysate. All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).

Immunoprecipitation of Beclin-1 from HeLa cells using Beclin-1 (D40C5) Rabbit mAb #3495 (lane 2). Lane 1 contains input control lysate. All lanes are probed with Beclin-1 (D40C5) Rabbit mAb #3495 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).免疫沉淀分析HeLa细胞的Beclin-1 ，所用检测抗体为Beclin-1 (D40C5) Rabbit mAb #3495 (泳道2)，泳道1包含对照组的提取物。所由泳道采用Beclin-1 (D40C5) Rabbit mAb #3495 作为一抗。二抗包括Anti-rabbit IgG, HRP-linked Anti- body #7074 (图 A) 和 Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记) #5127（图B）.

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货号：

5127S

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询价

100ul

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