首页抗体二抗抗小鼠

BD Pharmingen™ PE Rat Anti-Mouse IgG1

1/2

BD Pharmingen™ PE Rat Anti-Mouse IgG1

品牌： BD Pharmingen

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

Mouse (QC Testing)

来源宿主：

Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ

展开

产品介绍

产品信息

耦联标记

宿主

Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ

免疫原

Pooled Mouse IgG1

简单描述

The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

商品描述

A85-1 The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

同种型

Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ

克隆号

克隆 A85-1 (RUO)

浓度

0.2 mg/ml

产品详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE

激发源:Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源:496 nm, 566 nm

最多发射源:576 nm

应用

实验应用

Flow cytometry (Routinely Tested)

反应种属

Mouse (QC Testing)

目标/特异性

IgG1

背景

别名

Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing ≤0.09% sodium azide.

文献

研发参考(3) 1. Bhattacharya D, Lee DU, Sha WC. Regulation of Ig class switch recombination by NF-kappaB: retroviral expression of RelB in activated B cells inhibits switching to IgG1, but not to IgE. Int Immunol. 2002; 14(9):983-991. (Clone-specific: Flow cytometry). 2. Honjo T, Obata M, Yamawaki-Katoaka Y, et al. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene. Cell. 1979; 18(2):559-568. (Biology). 3. Ozaki K, Spolski R, Ettinger R, et al. Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6. J Immunol. 2004; 173(9):5361-5371. (Clone-specific: Flow cytometry).

数据库链接

Entrez-Gene ID

16017

参考图片

Flow cytometric analysis of CD22.2 expression by mouse splenocytes using PE Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were stained with either Purified Mouse IgG1, κ Anti-Mouse CD22.2 antibody (clone Cy34.1; solid line histogram) or with no antibody (for background staining control; dashed line histogram). After washing, the cells were stained with PE Rat Anti-Mouse IgG1 antibody (Cat. No. 550083) as the second step at 1 µg/test. The fluorescence histogram showing CD22.2 expression (or control staining) as detected by PE Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and analysis was performed using a BD™ LSR II Flow Cytometer System and FlowJo™ software.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

550083

一键复制

询价

优宁维现货

25ug

100ug

选择数量

当前规格1件起购

配送至

预计3-4个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价