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品牌: BD Pharmingen
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实验应用:
Flow cytometry, Isotype control, Intracellular staining (flow cytometry) (Routinely Tested)
Flow cytometry, Isotype control, Intracellular staining (flow cytometry) (Routinely Tested)
产品介绍
产品信息
耦联标记
PE
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免疫原
Mouse immunoglobulin
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简单描述
Following immunization of a rat with mouse immunoglobulin (Ig), the Ig from the R3-34 hybridoma was identified as a non-reactive clone. The R3-34 immunoglobulin was selected as an Ig isotype control following screening for low background staining on a variety of mouse and human cells and tissues.
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同种型
Rat IgG1, κ
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克隆号
R3-34
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浓度
0.2 mg/ml
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产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry, Isotype control, Intracellular staining (flow cytometry) (Routinely Tested)
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制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
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参考图片
Expression of IL-6 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS (100 ng/ml) in the presence of 2 µM GolgiStop™ (Cat. No. 554724). The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and sbsequently stained with 0.25 µg of PE-rat IgG1 anti-human IL-6 antibody (PE-MQ2-13A5, Cat. No. 554545; left panel) or 0.25 µg of PE-R3-34 immunoglobulin (right panel), following BD Biosciences Pharmingen's staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals.
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