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Alexa Fluor 647 Mouse IgG1 κ Isotype Control(MOPC-21)

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Alexa Fluor 647 Mouse IgG1 κ Isotype Control(MOPC-21)

品牌： BD Pharmingen

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参数

实验应用：

Intracellular staining (flow cytometry), Isotype control (Routinely Tested)

产品介绍

产品信息

耦联标记

Alexa Fluor 647

简单描述

The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

同种型

Mouse IgG1, κ

克隆号

MOPC-21

产品详情

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

应用

实验应用

Intracellular staining (flow cytometry), Isotype control (Routinely Tested)

推荐用量

5 µl

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(1) 1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Clone-specific: Blocking, Flow cytometry).

参考图片

Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA (5 ng/ml, Sigma, Cat. No. P-8139) in the presence of Brefeldin A (BD GolgiPlug™ Protein Transport Inhibitor, Cat. No. 555029). Cells were harvested, fixed, permeabilized and stained with PE Mouse Anti-Human CD8 (Cat. No. 555367) and either Alexa Fluor®647 Mouse Anti Human TNF (Cat. No. 557733; left panel) or Alexa Fluor® 647 MOPC-21 immunoglobulin (Cat. No. 557732, right panel). Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls.

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货号：

557732

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