Anti-Mouse IgG1 Magnetic Particles - DM(A85-1)

Figure 1. Depletion of human T and B lymphocytes and NK cells. Human PBMC were stained with APC Mouse Anti-Human CD4 mAb RPA-T4 (Cat. No. 555349), CD8 mAb RPA-T8 (Cat. No. 555369), CD19 mAb HIB19 (Cat. No. 555415), and CD56 mAb B159 (Cat. No. 555518), then labeled with BD IMag™ Anti-Mouse IgG1 Magnetic Particles - DM (Cat. No. 557983). After labeling, the cells were separated using the BD IMag™ Cell Separation Magnet, and the depleted and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Depletion Flow Chart to identify the separated cell populations represented in this figure. Unseparated PBMC (left panel), the final depleted fraction (middle panel), and the positive fraction (right panel) were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with Propidium Iodide Staining Solution (Cat. No. 557983), and mononuclear cells were identified by scatter profile. The percentage of non-T-B-NK leukocytes in each sample is given. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Figure 2. Positive selection of rat αß TCR-expressing T lymphocytes. Lewis rat splenocytes were stained with FITC Mouse Anti-Rat αß TCR mAb R73 (Cat. No. 554913), and then labeled with BD IMag™ Anti-Mouse IgG1 Magnetic Particles - DM. After labeling, the cells were separated using the BD IMag™ Cell Separation Magnet, and the negative and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. Unseparated Spleen (left panel), the Negative Fraction (middle panel), and the Positive Fraction (right panel) were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with propidium iodide, and all viable leukocytes are displayed. The percentage of αβ TCR+ T lymphocytes in each sample i