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产品介绍
产品信息
抗原名称
Monocyte/NK

克隆号
(RUO)

组合货号
51-9007391AK/(2 to 8C)+51-9007391BK/室温

应用
反应种属
Human (QC Testing)

目标/特异性
CD14, CD19, CD16, CD56, CD3, ERK1/2 (pT202/pY204), p38 MAPK (pT180/pY182), Stat1 (pY701), Stat3 (pY705), Stat5 (pY694), Stat6 (pY641)

文献
文献
研发参考(10)
1. Covey TM, Putta S, Cesano A. Single cell network profiling (SCNP): mapping drug and target interactions. Assay Drug Dev Technol. 2010; 8:321–343. (Biology).
2. Critchley-Thorne RJ, Simons DL, Yan N, et al. Impaired interferon signaling is a common immune defect in human cancer. Proc Natl Acad Sci U S A. 2009; 106:9010–9015. (Biology).
3. Irish JM, Kotecha N, Nolan GP. Mapping normal and cancer cell signalling networks: towards single-cell proteomics. Nat Rev Cancer. 2006; 6:146-155. (Biology).
4. Krutzik PO, Crane JM, Clutter MR, Nolan GP. High-content single-cell drug screening with phosphospecific flow cytometry. Nat Chem Biol. 2008; 4(2):132-142. (Biology).
5. Lee AW, Sharp ER, O'Mahony A, et al. Single-cell, phosphoepitope-specific analysis demonstrates cell type- and pathway-specific dysregulation of Jak/STAT and MAPK signaling associated with in vivo human immunodeficiency virus type 1 infection. J Virol. 2008; 82:3702–3712. (Biology).
6. Montag DT, Lotze MT. Rapid flow cytometric measurement of cytokine-induced phosphorylation pathways [CIPP] in human peripheral blood leukocytes. Clin Immunol. 2006; 121(2):215-226. (Biology).
7. Montag DT, Lotze MT. Successful simultaneous measurement of cell membrane and cytokine induced phosphorylation pathways [CIPP] in human peripheral blood mononuclear cells. J Immunol Methods. 2006; 313(1-2):48-60. (Biology).
8. Perez OD, Krutzik PO, Nolan GP. Flow cytometric analysis of kinase signaling cascades. Methods Mol Biol. 2004; 263:67–94. (Biology).
9. Perez OD, Mitchell D, Campos R, Gao GJ, Li L, Nolan GP. Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry. Curr Protoc Cytom. 2005; 6.20.1-6.20.22. (Biology).
10. Schulz KR, Danna EA, Krutzik PO, Nolan GP. Single-cell phospho-protein analysis by flow cytometry. Curr Protoc Immunol. 2007. (Biology).

参考图片
Effects of cytokine stimulation on phosphorylation of various STAT molecules in monocyte and NK cell subsets.
Effects of cytokine stimulation on phosphorylation of various STAT molecules in monocyte and NK cell subsets.s
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