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rcynoIgG1 Fc, CF (100 ug)
rcynoIgG1 Fc, CF (100 ug)

rcynoIgG1 Fc, CF (100 ug)

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品牌： R&D Systems
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    产品介绍
    产品信息

    Scientific Data

    Bioactivity Recombinant Cynomolgus Monkey IgG1 Fc Protein BioactivityView Larger

    When Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 µg/mL, 100 µL/well, Recombinant Human Fc gamma RI/CD64 (Catalog # 1257-FC) binds with an ED50 of 0.2-1.2 ng/mL.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    9315-HG

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 1 mg/mL in PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey IgG1 Fc is immobilized at 0.15 µg/mL (100 µL/well), the concentration of Recombinant Human Fc gamma  RI/CD64 (Catalog # 1257-FC) that produces 50% of the optimal binding response is 0.2-1.2 ng/mL.
    Source
    Human embryonic kidney cell, HEK293-derived cynomolgus monkey IgG1 protein
    Pro110-Lys333
    Accession #
    AAF14057
    N-terminal Sequence
    Analysis
    Pro110, and Ala-Leu-Ala-Pro110
    Predicted Molecular Mass
    25 kDa
    SDS-PAGE
    30-40 kDa, reducing conditions
    背景
    别名
    IgG1,Igh-4,RGD1359539,VH7183,Immunoglobulin G1
    背景

    Background: IgG1

    Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in human adult blood. It is composed of two heavy chains and two light chains. The variability of the IgG pool is generated by somatic recombination in B cells that have been exposed to foreign material. IgA, IgG, IgM, IgD, and IgD classes of human immunoglobulin (as well as the isotypes IgG1, IgG2a, IgG2b, and IgG3) are generated by class switch recombination within the heavy chain locus (1). Recombination within the light chain locus selects either kappa or lambda chains. Antibodies are secreted by plasma cells of the B lymphocyte lineage and constitute the humoral component of adaptive immunity (2, 3). Their variable regions bind to foreign antigens, while their Fc regions are recognized by Fc receptors expressed on macrophages, monocytes, neutrophils, NK cells, and CD8+ cytotoxic T cells (4). Phagocyte uptake of antibody-antigen complexes and antibody-opsonized pathogens is critical for clearance of the foreign material and immune homeostasis.

    References
    1. Stavnezer, J. et al. (2008) Annu. Rev. Immunol. 26:261.
    2. Nutt, S.L. et al. (2015) Nat. Rev. Immunol. 15:160.
    3. Swanson, C.L. et al. (2013) Immunol. Res. 55:277.
    4. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
    Long Name
    Immunoglobulin G1
    Alternate Names
    IgG1; Igh-4; RGD1359539; VH7183
    制备和贮存
    溶解方法
    Reconstitute at 1 mg/mL in PBS.

    参考图片

    When Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 μg/mL, 100 μL/well, Recombinant Human Fc gamma  RI/CD64 (Catalog # 1257-FC) binds with an ED50 of 0.2-1.2 ng/mL.

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    货号：
    9315-HG-100
    一键复制
    询价
    100ug
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