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品牌: BD Pharmingen
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实验应用:
Isotype control, Flow cytometry (Routinely Tested)
Isotype control, Flow cytometry (Routinely Tested)
产品介绍
产品信息
耦联标记
BV421
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免疫原
TNP-keyhole limpet hemocyanin
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简单描述
The G155-178 clone has an unknown specificity. Trinitrophenol (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to immunoglobulin Fc receptors. The immunoglobulin secreted by the G155-178 hybridoma was selected as a mouse IgG2a, κ isotype control following screening for low background binding on a variety of mouse and human tissues.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
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同种型
Mouse BALB/c IgG2a, κ
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克隆号
G155-178
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浓度
0.2 mg/ml
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产品详情
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
应用
实验应用
Isotype control, Flow cytometry (Routinely Tested)
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背景
别名
IgG2a kappa Isotype Control
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制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
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参考图片
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