BD Horizon™ BV421 Rat IgG2a, κ Isotype Control
品牌： BD Pharmingen
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咨询实验应用：
Flow cytometry, Isotype control (Routinely Tested)
Flow cytometry, Isotype control (Routinely Tested)
产品介绍
产品信息
耦联标记
BV421
免疫原
Mouse Pooled Immunoglobulin
简单描述
The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
克隆号
克隆 R35-95 (RUO)
浓度
0.2 mg/ml
产品详情
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
Violet 405 nm
407 nm
423 nm
应用
实验应用
Flow cytometry, Isotype control (Routinely Tested)
目标/特异性
Rat IgG2a κ Isotype Ctrl
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考图片
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