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Mouse (E5Y6Q) mAb IgG2a Isotype Control
Mouse (E5Y6Q) mAb IgG2a Isotype Control

Mouse (E5Y6Q) mAb IgG2a Isotype Control

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品牌： CST
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    来源宿主：
    Mouse
    Mouse
    产品介绍
    产品信息
    来源纯化
    人碱性 FGF 重组蛋白在大肠杆菌中表达，并且以冻干形式保存。
    宿主
    Mouse
    简单描述
    Monoclonal Antibody for studying Mouse IgG isotype control. Cited in 20 publications. Validated for IP, IHC, IF, IF, F, F, ChIP. Highly specific and rigorously validated in-house, Mouse (E5Y6Q) Monoclonal Antibody IgG2a Isotype Control (CST #61656) is ready to ship.
    商品描述

    Product Usage Information

    Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.

    浓度
    2.5 mg/ml
    应用
    目标/特异性

    Specificity/Sensitivity

    Mouse (E5Y6Q) mAb IgG2a Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG2a monoclonal antibodies.

    敏感性
    Endogenous
    背景
    背景
    Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    参考图片

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using PCNA (PC10) Mouse mAb (left) compared to concentration matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (right).

    Confocal immunofluorescent analysis of HCT 116 cells using CD44 (156-3C11) Mouse mAb #3570 (left, green) compared to concentration matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (right, green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

    Flow cytometric analysis of unpermeabilized HeLa cells using CD44 (156-3C11) Mouse mAb #3570 (solid line) compared to concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (dashed line). Anti-mouse IgG (H+L) F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mouse embryonic fibroblasts treated with tunicamycin (2ug/ml, 10hr) and either Mouse (E5Y6Q) mAb IgG2a Isotype Control, Normal Rabbit IgG #2729, or CHOP (L63F7) Mouse mAb #2895 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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    货号：
    61656S
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    询价
    供应商现货
    100ul
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    当前规格1件起购 
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