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hIL-2 QKit, 2nd Gen (1 Kit)
hIL-2 QKit, 2nd Gen (1 Kit)
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hIL-2 QKit, 2nd Gen (1 Kit)

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品牌： R&D Systems
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    产品介绍
    产品信息

    Recovery

    The recovery of human TNF-alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

    Sample TypeAverage % RecoveryRange %
    Cell Culture Media (n=5)10196-107
    Citrate Plasma (n=5)10598-111
    EDTA Plasma (n=5)118104-139
    Heparin Plasma (n=5)10994-129
    Serum (n=5)9989-114

    Linearity

    To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TNF- alpha in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
    Human IL-2 ELISA Linearity

    Scientific Data

    N/A Human IL-2 ELISA Standard Curve for Cell Culture Supernate AssayView Larger

    Human IL-2 ELISA Standard Curve for Cell Culture Supernate Assay

    N/A Human IL-2 ELISA Standard Curve for Serum/Plasma AssayView Larger

    Human IL-2 ELISA Standard Curve for Serum/Plasma Assay

    Assay Procedure

    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
      3. 100 µL Assay Diluent
    3.   Add 100 µL of Assay Diluent to each well.
      4. 100 µL Standard, Control, or Sample
    4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
      6. 200 µL Conjugate
    6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate and wash 3 times.
      8. 200 µL Substrate Solution
    8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
      9. 50 µL Stop Solution
    9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    Human IL-2 Quantikine ELISA Kit Summary

    Assay Type
    Solid Phase Sandwich ELISA
    Format
    96-well strip plate
    Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
    Sensitivity
    7 pg/mL
    Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
    Specificity
    Natural and recombinant human TNF- alpha.
    Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
    Interference
    No significant interference observed with available related molecules.
    背景
    别名
    Aldesleukin,IL2,IL-2,IL-2lymphokine,interleukin 2,interleukin-2,involved in regulation of T-cell clonal expansion,Proleukin,T cell growth factor,T-cell growth factor,TCGF,Interleukin 2
    背景

    Background: IL-2

    Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha -helical polypeptide that is a member of the Common gamma Chain ( gamma c) cytokine family (1-4). It exists as a monomer and has a notably short half-life (< 30 minutes) (1). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region (5, 6). The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity (7). Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells (8), activated conventional CD4+ and CD8+ T cells (1, 9), neurons (10, 11), microglia (12), and hematopoietic stem cells (13). 

    The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3). 
    In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).
    Long Name:
    Interleukin 2
    Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
    Alternate Names:
    Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF
    研究领域
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    货号：
    D2050
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