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IL-6 (D5W4V) XP ® Rabbit mAb
IL-6 (D5W4V) XP ® Rabbit mAb
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IL-6 (D5W4V) XP ® Rabbit mAb

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品牌： CST
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    分子量：
    24
    24
    反应种属：
    Mouse
    Mouse
    展开
    产品介绍
    产品信息
    抗原名称
    IL-6
    来源纯化
    使用重组小鼠 IL-6 蛋白对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying IL6 mouse. Cited in 188 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-6 (D5W4V) XP® Rabbit Monoclonal Antibody (CST #12912) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:100
    Immunofluorescence (Immunocytochemistry)1:200 - 1:400
    Flow Cytometry (Fixed/Permeabilized)1:400
    分子量
    24
    研究领域
    癌症,纤维化,神经科学
    应用
    反应种属
    Mouse
    目标/特异性

    Specificity/Sensitivity

    IL-6 (D5W4V) XP® Rabbit mAb 可识别 IL-6 总蛋白的内源水平。

    物种反应性：

    小鼠

    敏感性
    Endogenous
    背景
    背景
    急性期反应由 T 细胞、巨噬细胞、成纤维细胞、内皮的和其他细胞产生的 interleukin-6 (IL-6) 引起 (1,2)。IL-6 诱导许多细胞类型的增殖或分化，包括 B 细胞、胸腺细胞和 T 细胞。与 TGF-β 一致，IL-6 对形成 Th17 反应很重要。IL-6 与 IL-6Rα 结合，并且通过这种结合 IL-6 可诱导 gp130 同型二聚化 (1)。gp130 同型二聚化可触发 Jak/Stat 级联和 SHP-2/ErkMAP 激酶级联 (1,3,4)。IL-6 还可与非膜结合的 IL-6Rα 剪接变体形成复合体 (3)。IL-6/可溶性 IL-6Rα 复合体随后可以在表达 gp130 但不表达 IL-6Rα 的细胞中激活 gp130 信号转导通路 (3)。调查研究已经表明通过增加促血管生成的 VEGF 表达，IL-6还可能导致转移性乳腺癌 (5)。 Heinrich, P.C. et al. (1998) Biochem J 334 ( Pt 2), 297-314. Heinrich, P.C. et al. (1998) Z Ernahrungswiss 37 Suppl 1, 43-9. Jones, S.A. (2005) J Immunol 175, 3463-8. Jenkins, B.J. et al. (2004) Mol Cell Biol 24, 1453-63. Hong, D.S. et al. (2007) Cancer 110, 1911-28.
    研究领域
    癌症,纤维化,神经科学
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #65145.
    数据库链接
    Entrez-Gene ID
    16193
    UniProt ID
    P08505

    参考图片

    Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left) or treated with LPS (100 ng/ml, 6 hr; right), using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

    Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment) (green), using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

    Immunoprecipitation of IL-6 from Raw 264.7 cell extracts treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific).

    Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation; +), using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Western blot analysis of recombinant Mouse His6Interleukin-6 (mHis6IL-6) #8857 using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific).

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    货号：
    12912S
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