Immunoprecipitation Kit: DYKDDDDK (FLAG®) for immunoprecipitation and purification of recombinant proteins containing the FLAG® epitope tag - KBA-319-383

Rockland Immunochemicals' Anti-DYKDDDDK (FLAG®) Kit for Immunoprecipitation allows for the purification by immunoprecipitation of recombinant proteins containing the FLAG® epitope tag provided by the user. The kit relies upon the high specificity of monoclonal antibody raised against the FLAG® epitope tag. This method is far easier and less costly than using antibodies produced against the recombinant protein itself therefore saving time and resources. Using the agarose bound antibody in this kit allows for efficient binding of FLAG® tag proteins without the need for preliminary steps or calibration. The immunoprecipitated FLAG® tag protein can be efficiently eluted from the agarose beads using a low pH elution step. The user is able to further characterize the resultant purified protein by size, post-translational modification, western blot and other assays.