
产品介绍
产品信息
宿主
鼠

克隆类别
单抗

Product Details
The 30H12 monoclonal antibody reacts with mouse Thy1.2 also known as CD90.2. Thy1.2 is expressed by thymocytes and mature T lymphocytes as well as hematopoietic stem cells, neurons, epithelial cells, and fibroblasts. Thy1.2 is expressed only by certain mouse strains including C57BL/6, BALB/c, CBA, C3H, C58/, SJL, DBA, and NZB/. Thy1.2 is a 25-35 kDa GPI-anchored membrane glycoprotein and a member of the immunoglobulin superfamily. The function of Thy1.2 has not been fully elucidated but is thought to play roles in cognition, axon growth, T lymphocyte function, and apoptosis. The 30H12 monoclonal antibody has been reported to induce Ca2+ flux in thymocytes. This antibody is particularly useful for depletion of T lymphocytes.Specifications
Isotype | Rat IgG2b, κ |
---|---|
Recommended Isotype Control(s) | InVivoPlus rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
Immunogen | Mouse thymus or spleen |
Reported Applications | in vivo ILC depletion in vivo T cell depletion Western blot |
Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin* | <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay |
Aggregation* | <5% Determined by SEC |
Purity | >95% Determined by SDS-PAGE |
Sterility | 0.2 μM filtered |
Production | Purified from cell culture supernatant in an animal-free facility |
Purification | Protein G |
RRID | AB_1107682 |
Molecular Weight | 150 kDa |
Murine Pathogen Tests* | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
组成成分
锚定膜蛋白抗体,磷酸盐缓冲液

应用
反应种属
mouse

背景
研究领域
Immunology, Neuroscience
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