ISRE Reporter BioAssay™ Kit (JAK/STAT Signaling Pathway)

HEK293

HEK293

Monitor IFNa-induced JAK/STAT pathway activity. Screen activators or inhibitors of the JAK/STAT signaling pathway. Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.

The ISRE Reporter BioAssay™ kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STATpathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.

Monitor IFNa-induced JAK/STAT pathway activity. Screen activators or inhibitors of the JAK/STAT signaling pathway. Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.

Tests500 Luminescent Cultured cells

38220000