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BD Horizon™ BV421 Armenian Hamster Anti-Mouse CD11c

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BD Horizon™ BV421 Armenian Hamster Anti-Mouse CD11c

品牌： BD Pharmingen

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反应种属：

Mouse (QC Testing)

来源宿主：

Armenian Hamster IgG2

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产品介绍

产品信息

耦联标记

BV421

抗原名称

CD11c

宿主

Armenian Hamster IgG2

免疫原

Mouse Dendritic Cells

简单描述

The N418 monoclonal antibody specifically binds to CD11c, the Integrin alpha X (Integrin αX, Itgax) chain of the heterodimeric gp150, 95 (CD11c/CD18, αXβ2) integrin that forms the complement receptor 4 (CR4). CD11c is a150 kDa type I transmembrane glycoprotein that is expressed on dendritic cells, CD4-CD8+ intestinal intraepithelial lymphocytes (IEL), and some NK cells and T cells. CD11c expression is upregulated on IEL and T cells following activation. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. The CD11c/CD18 integrin can bind to several ligands including iC3b, fibrinogen, and CD54. This integrin reportedly plays important roles in phagocytosis and in mediating cellular interactions during inflammation.

商品描述

N418 The N418 monoclonal antibody specifically binds to CD11c, the Integrin alpha X (Integrin αX, Itgax) chain of the heterodimeric gp150, 95 (CD11c/CD18, αXβ2) integrin that forms the complement receptor 4 (CR4). CD11c is a150 kDa type I transmembrane glycoprotein that is expressed on dendritic cells, CD4-CD8+ intestinal intraepithelial lymphocytes (IEL), and some NK cells and T cells. CD11c expression is upregulated on IEL and T cells following activation. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. The CD11c/CD18 integrin can bind to several ligands including iC3b, fibrinogen, and CD54. This integrin reportedly plays important roles in phagocytosis and in mediating cellular interactions during inflammation.

同种型

Armenian Hamster IgG2

克隆号

克隆 N418 (RUO)

浓度

0.2 mg/ml

产品详情

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:BV421

激发源:Violet 405 nm

最多激发源:407 nm

最多发射源:423 nm

应用

实验应用

Flow cytometry (Routinely Tested)

反应种属

Mouse (QC Testing)

目标/特异性

CD11c (Integrin αX)

背景

别名

Itgax; Integrin alpha-X; Integrin αX; ITAX; CR4; Complement Receptor 4

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(3) 1. Crowley MT, Inaba K, Witmer-Pack MD, Gezelter S, Steinman RM. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Methods. 1990; 133(1):55-66. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). 2. Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990; 171(5):1753-1771. (Immunogen: Flow cytometry, Immunohistochemistry, Immunoprecipitation). 3. Sadhu C, Ting HJ, Lipsky B, et al. CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007; 81(6):1395-1403. (Clone-specific: Blocking).

参考图片

Flow cytometric analysis of CD11c expression on C57BL/6 mouse splenic leucocyte subsets. Panel 1. Splenic Leucocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with Alexa Fluor® 647 Rat Anti-Mouse I-A/I-E (Cat. No. 562367) and either BD Horizon™ BV421 Hamster IgG2, λ Isotype Control (Cat. No. 562629; Left Plot) or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody (Cat. No. 565451/565452; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of I-A/I-E versus CD11c (or Ig Isotype control staining) were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Panel 2. Ly6G/Ly6C-negative Splenic Leucocytes. Mouse splenic leucocytes were similarly preincubated with Mouse BD Fc Block™ and stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092/561880) and PerCP-Cy™5.5 Rat Anti-Mouse Ly6G/Ly6C (Cat. No. 552093) antibodies and either BD Horizon BV421 Hamster IgG2, λ Isotype Control (Left Plot) or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody (Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD45R/B220 versus CD11c (or Ig Isotype control staining) were derived from Ly6G/Ly6C- events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer system.

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货号：

565452

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