BD Pharmingen™ Purified Rat Anti-Mouse CD29

CD29 (Integrin β1)

Rat IgG2a, κ

Mouse endothelial cell line

The 9EG7 antibody reacts with the 130-kDa integrin β1 chain (CD29).  CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin.  It  also associates with the integrin α7 α8 and α9 chains in non-lymphoid tissues.  As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia.  9EG7 mAb has been shown to inhibit both the α6β1-mediated binding of lymphocytes to endothelial cells and the adhesion  mediated by activated, but not unactivated, α4β1-integrin.  The source of the immunogen was mouse lymph node-derived endothelial cell line TME.

The 9EG7 antibody reacts with the 130-kDa integrin β1 chain (CD29).  CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin.  It  also associates with the integrin α7 α8 and α9 chains in non-lymphoid tissues.  As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia.  9EG7 mAb has been shown to inhibit both the α6β1-mediated binding of lymphocytes to endothelial cells and the adhesion  mediated by activated, but not unactivated, α4β1-integrin.  The source of the immunogen was mouse lymph node-derived endothelial cell line TME.

Rat IgG2a, κ

克隆 9EG7 (RUO)

0.5 mg/ml

Flow cytometry (Routinely Tested), Immunohistochemistry-paraffin (Tested During Development), Functional assay, Immunoprecipitation, Western blot (Reported)

Mouse (QC Testing)

CD29 (Integrin β1)

Integrin β1 chain

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(9) 1. Driessens MH, Van Rijthoven EA, Kemperman H, Roos E. Adhesion of lymphoma cells to fibronectin: differential use of alpha 4 beta 1 and alpha 5 beta 1 integrins and stimulation by the 9EG7 mAb against the murine beta 1 integrin subunit. Cell Adhes Commun. 1995; 3(4):327-336. (Clone-specific: Functional assay). 2. Kassner PD, Kawaguchi S, Hemler ME. Minimum alpha chain cytoplasmic tail sequence needed to support integrin-mediated adhesion. J Biol Chem. 1994; 269(31):19859-19867. (Clone-specific: Functional assay). 3. Lenter M, Uhlig H, Hamann A, Jenö P, Imhof B, Vestweber D. A monoclonal antibody against an activation epitope on mouse integrin chain beta 1 blocks adhesion of lymphocytes to the endothelial integrin alpha 6 beta 1. Proc Natl Acad Sci U S A. 1993; 90(19):9051-9055. (Immunogen: Functional assay, Immunoprecipitation). 4. Lenter M, Vestweber D. The integrin chains beta 1 and alpha 6 associate with the chaperone calnexin prior to integrin assembly. J Biol Chem. 1994; 269(16):12263-12268. (Clone-specific: Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Western blot). 5. Milner R, Edwards G, Streuli C, Ffrench-Constant C. A role in migration for the alpha V beta 1 integrin expressed on oligodendrocyte precursors. J Neurosci. 1996; 16(22):7240-7252. (Clone-specific: Functional assay, Immunoprecipitation). 6. Mouse Genome Database (MGD), Mouse Genome Informatics, The Jackson Laboratory. B2m, beta-2 microglobulin. Available: http://www.informatics.jax.org 4/15/1998. 7. Rich S, Van Nood N, Lee HM. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996; 157(7):2916-2923. (Clone-specific: Functional assay). 8. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Clone-specific: Immunoprecipitation). 9. Yashiro Y, Tai XG, Toyo-oka K, et al. A fundamental difference in the capacity to induce proliferation of naive T cells between CD28 and other co-stimulatory molecules. Eur J Immunol. 1998; 28(3):926-935. (Biology).