ITS (100X) Media Sup (5 mL)

品牌： R&D Systems

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

产品介绍

产品信息

试剂盒组分

5 mL ITS Media Supplement (100x)

Component	Amount (µg/mL)
Recombinant Human Insulin	1000
Human Transferrin	500
Selenite	0.67

Stability and Storage

Store in the dark at < -20 ^°C in a manual defrost freezer. Do not use past the expiration date.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.

Limitations

FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
This reagent should not be used beyond the expiration date indicated on the label.

Scientific Data

Cell Proliferation

View Larger

ITS Media Supplement Supports Proliferation Under Low Serum Conditions. ITS Media Supplement Supports Proliferation Under Low Serum Conditions.Hela, MCF7, and HepG2 cells were cultured in media supplemented with 10% FBS, low serum (0.1% FBS), or ITS Media Supplement + 0.1% FBS. Cells were trypsinized, harvested, and cell counts were performed. For each respective cell line, cell counts were normalized against standard 10% FBS-containing media (100%).

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, ITS Media Supplement is prepared using the following procedure:

Dilute the media into the cell culture media of interest
Store completed media at 2 – 8 °C

Reagents Provided

Reagents provided in the ITS Media Supplement (100X) (Catalog # AR013):

Component	Amount (µg/mL)
Recombinant Human Insulin	1000
Human Transferrin	500
Selenite	0.67

Stability and Storage

Store in the dark at < -20 ^°C in a manual defrost freezer. Do not use past the expiration date.

Precautions

Other Supplies Required

Materials

Basal media
Serological pipettes
Pipettes and pipette tips

Equipment

2 °C to 8 °C refrigerator

Procedure Overview

Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.

ITS Media Supplement (100X) Summary

Kit Summary

Supports cell proliferation under low serum conditions.

Key Benefits

Contains optimized concentrations of insulin, transferrin, and selenite
Chemically-defined media decreases experimental variation
High lot-to-lot consistency decreases variation
Performance tested on multiple cell lines

Why Use Pre-optimized and Serum-free Supplements for Cell Culture?

The efficiency of cell proliferation and maintenance of cell health can vary from one experiment to another depending on the media components, the supplements used to drive proliferation, and the quality of the starting cell population.

Although commonly used, serum-containing medium is inherently variable due to the undefined composition and lot-to-lot variability across batches of serum. Troubleshooting media variability can be a costly and time-consuming obstacle that can be minimized through the use of high quality media. Our ITS Media Supplement is a pre-optimized and serum-free media supplement that was designed to help maintain culture media consistency across passages and experiments. The ITS Media Supplement contains optimized concentrations of human insulin, human transferrin, and selenite.

ITS Media Supplement (100x):

Contains optimized concentrations of insulin, transferrin, and selenite
Chemically-defined media decreases experimental variation
High lot-to-lot consistency decreases variation
QC-tested on multiple cell lines

Kit Contents

5 mL ITS Media Supplement (100x)

Component	Amount (µg/mL)
Recombinant Human Insulin	1000
Human Transferrin	500
Selenite	0.67

Stability and Storage

Store in the dark at < -20 ^°C in a manual defrost freezer. Do not use past the expiration date.

Precautions

Limitations

FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
This reagent should not be used beyond the expiration date indicated on the label.

Specifications

Shipping Conditions

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Species

Multi-Species

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

AR013

一键复制

询价

5mL

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价