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Flow cytometric analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究K-562细胞,所用抗体为Jak2 (D2E12) XP® Rabbit mAb (蓝色) 与非特异性的对照抗体(红色)。
Confocal immunofluorescent analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).共聚焦免疫荧光分析K-562 细胞,所用抗体为 Jak2 (D2E12) XP® Rabbit mAb (绿色)。 Blue pseudocolor = DRAQ5® (DNA 荧光染料)。
Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP® Rabbit mAb.Western免疫印迹。用Jak2 (D2E12) XP® Rabbit mAb研究K-562, THP-1, TF-1 和 BaF3 细胞系的细胞提取液。
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为Jak2 (D2E12) XP® Rabbit mA 。
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).免疫组织化学染色分析石蜡包埋人肺癌组织。在对照多肽(左图) 或 Jak2 封闭多肽(右图)存在下所用抗体为Jak2 (D2E12) XP® Rabbit mA 。
Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.Western免疫印迹。用Jak2 (D2E12) XP® Rabbit mAb #3230 和 α-Tubulin (11H10) Rabbit mAb #2125研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或Jak2 siRNA (+)的K-562细胞的细胞提取液。Jak2 (D2E12) XP® Rabbit mAb 证实对Jak2 表达的沉默, α-Tubulin (11H10) Rabbit mAb作为上样量和Jak2 siRNA特异性的对照。