
产品介绍
产品信息
简单描述
siRNA for studying PKA C-alpha in the research area.

商品描述
Product Usage Information
CST recommends transfection with 100 nM PKA C-α siRNA 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

研究领域
细胞生物学,纤维化,代谢,神经科学,

应用
反应种属
Human

背景
背景
The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (2). Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9) (3-5). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259) (6). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197 (7).
1.Montminy, M. (1997) Annu. Rev. Biochem. 66, 807-822.
2.Dell'Acqua, M.L. and Scott, J.D. (1997) J. Biol. Chem. 272, 12881-12884.
3.Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
4.Gonzalez, G.A. and Montminy, M.R. (1989) Cell 59, 675-680.
5.Fang, X. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 11960-11965.
6.Dumaz, N. and Marais, R. (2003) J. Biol. Chem. 278, 29819 -29823.
7.Moore, M.J. et al. (2002) J. Biol. Chem. 277, 47878-47884.

研究领域
细胞生物学,纤维化,代谢,神经科学,
制备和贮存
保存方式
SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
数据库链接
Entrez-Gene ID
5566

UniProt ID
P17612

参考图片
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