Ki67 Recombinant Mouse mAb (SDT-606-7)
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Ki67 Recombinant Mouse mAb (SDT-606-7)

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品牌: STARTER
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    反应种属:
    Hu
    Hu
    来源宿主:
    Mouse
    Mouse
    展开
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein G
    抗原名称
    Ki67
    宿主
    Mouse
    免疫原
    Synthetic Peptide
    同种型
    IgG1
    克隆号
    SDT-606-7
    浓度
    2 mg/ml
    性状
    Liquid
    缓冲体系
    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
    产品类型
    Recombinant mAb
    应用
    实验应用
    IHC-P、ICC、IF
    反应种属
    Hu
    稀释度
    • IHC

      1:250
    • ICC

      1:500
    • IF

      1:200
    背景
    别名
    Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, MKI67
    背景

    The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy.

    细胞定位
    Nucleus
    制备和贮存
    保存方式

    12 months from date of receipt / reconstitution, -20°C as supplied

    数据库链接
    Accession

    参考图片

    IHC shows positive staining in paraffin-embedded human tonsil. Anti-Ki67 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human spleen. Anti-Ki67 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human colon. Anti-Ki67 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Ki67 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Ki67 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Ki67 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Ki67 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    ICC shows positive staining in HeLa cells. Anti-Ki67 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    ICC shows positive staining in HeLa cells. Anti-Ki67 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).

    ICC shows positive staining in HT-29 cells. Anti-Ki67 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).

    IF shows positive staining in paraffin-embedded human tonsil. Anti-Ki67 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    IF shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Ki67 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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    货号:
    S0B2259-1ml
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