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Product Summary
The Parameter beta 2-Microglobulin Immunoassay is a 1.5 hour forward sequential competitive enzyme immunoassay designed to measure beta 2M in serum, plasma, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three controls of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three controls of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (µg/mL) | 1.14 | 3.19 | 6.41 | 0.953 | 3.1 | 6.17 |
| Standard Deviation | 0.086 | 0.189 | 0.383 | 0.175 | 0.293 | 0.496 |
| CV% | 7.5 | 5.9 | 6 | 18.4 | 9.5 | 8 |
Recovery
The recovery of human beta 2M spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 99 | 79-114 |
| Heparin Plasma (n=4) | 106 | 91-124 |
| Serum (n=4) | 101 | 85-122 |
| Urine (n=4) | 90 | 76-105 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human beta 2M were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Human beta 2-Microglobulin Parameter Assay Kit Summary
Assay Length
1.5 hours
Sample Type & Volume Required Per Well
Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Urine (10 uL)
Sensitivity
0.132 ug/mL
Assay Range
0.2 - 10 ug/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Measures Human beta 2M levels in various samples.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
背景
别名
B2M,beta 2Microglobulin,beta 2-Microglobulin,beta chain of MHC class I molecules,beta-2-microglobin,beta-2-microglobulin
背景
Background: beta 2-Microglobulin
beta 2-Microglobulin (B2M) forms the light chain of Class I MHC molecules. It has been measured in a variety of body fluids, including serum/plasma, saliva, CSF, and urine. B2M freely passes through the glomerular membrane, but it is nearly completely reabsorbed and degraded in proximal tubule cells. Clinically, serum B2M is elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions associated with decreased glomerular filtration.
Entrez Gene IDs:
567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)
Alternate Names:
B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
研究领域
制备和贮存
保存方式
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
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危险品化学品经营许可证(不带存储) 许可证编号：沪(杨)应急管危经许[2022]202944(QY) 
营业执照(三证合一)