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rmKLRG1 Fc, CF (50 ug)
rmKLRG1 Fc, CF (50 ug)

rmKLRG1 Fc, CF (50 ug)

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    Scientific Data

    Bioactivity Recombinant Mouse KLRG1 Fc Chimera Protein BioactivityView Larger

    When Recombinant Mouse E-Cadherin (Catalog # 8875-EC) is coated at 5 µg/mL, 100 µL/well, Recombinant Mouse KLRG1 Fc Chimera (Catalog # 6944-KR) binds with an ED50 of 15-90 ng/mL.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    6944-KR

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 200 μg/mL in PBS.
    ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Mouse KLRG1 Fc Chimera Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse E-Cadherin (Catalog # 8875-EC) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse KLRG1 Fc Chimera that produces 50% of the optimal binding response is 15-90 ng/mL.
    Source
    Chinese Hamster Ovary cell line, CHO-derived mouse KLRG1 protein
    MDPMouse IgG2A
    (Glu98-Lys330)    		IEGRMouse KLRG1
    (Gln57-Tyr188)
    Accession # O88713
    N-terminusC-terminus
    Accession #
    O88713
    N-terminal Sequence
    Analysis
    Met
    Structure / Form
    Disulfide-linked homodimer
    Predicted Molecular Mass
    42.2 kDa (monomer)
    SDS-PAGE
    45-57 kDa, reducing conditions
    背景
    别名
    2F1,CLEC15A,CLEC15AMAFA-LIKE,C-type lectin domain family 15 member A,C-type lectin domain family 15, member A,ITIM-containing receptor MAFA-L,killer cell lectin-like receptor subfamily G member 1,killer cell lectin-like receptor subfamily G, member 1,KLRG1,MAFA,MAFAL,MAFA-L,MAFA-like receptor,MAFAMAFA-2F1,mast cell function-associated antigen (ITIM-containing),Mast cell function-associated antigen,MGC13600,Killer Cell Lectin-like Receptor Subfamily G Member 1
    背景

    Background: KLRG1

    KLRG1 (killer cell lectin‑like receptor G1), also called MAFA (mast cell function associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one immunoreceptor tyrosine‑based inhibitory motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8+, CD4+, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8+ T cells in the elderly and by virus‑specific CD8+ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

    References
    1. Henson, S.M. and A.N. Akbar (2009) Age 31:285.
    2. Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
    3. Voehringer, D. et al. (2001) Immunogenetics 52:206.
    4. Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
    5. Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
    6. Voehringer, D. et al. (2002) Blood 100:3698.
    7. Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
    8. Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
    9. Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
    10. Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
    11. Thimme, R. et al. (2005) J. Virol. 79:12112.
    12. Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
    13. Ito, M. et al. (2006) J. Exp. Med. 203:289.
    14. Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
    15. Banh, C. et al. (2009) Blood 114:5299.
    Long Name
    Killer Cell Lectin-like Receptor Subfamily G Member 1
    Entrez Gene IDs
    10219 (Human); 50928 (Mouse); 58975 (Rat)
    Alternate Names
    2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 100 μg/mL in PBS.

    参考图片

    WhenRecombinant Mouse E‑Cadherin (Catalog # 8875‑EC) is coated at 5 µg/mL, 100 μL/well, Recombinant Mouse KLRG1 FcChimera (Catalog # 6944‑KR) binds withan ED50 of 15‑90 ng/mL.

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    货号：
    6944-KR-050
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    50ug
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