LDL receptor Recombinant Rabbit  mAb (SDT-057-57)
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LDL receptor Recombinant Rabbit mAb (SDT-057-57)

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品牌: STARTER
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    反应种属:
    Hu、Ms、Rt
    Hu、Ms、Rt
    来源宿主:
    Rabbit
    Rabbit
    展开
    产品介绍
    产品信息
    纯化方式
    Protein A
    抗原名称
    LDL Receptor
    宿主
    Rabbit
    免疫原
    Synthetic Peptide
    克隆号
    SDT-057-57
    浓度
    0.25mg/ml
    性状
    Liquid
    缓冲体系
    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
    产品类型
    Rabbit mAb
    应用
    实验应用
    WB、IHC-P
    反应种属
    Hu、Ms、Rt
    预测反应种属
    Bv, Rb
    稀释度
    • IHC-P

      1:1000
    • WB

      1:500
    背景
    别名
    Low-density lipoprotein receptor, LDLR
    背景

    The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors.Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis.LDLR is ubiquitously expressed and is a key receptor for maintaining cholesterol homeostasis in mammals. LDLR-mediated endocytosis is essential for lipoprotein and lipid metabolism.Impaired LDLR function by genetic mutations results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

    细胞定位
    Golgi Apparatus、Lysosome、Endosome、Plasma Membrane
    制备和贮存
    保存方式

    12 months from date of receipt / reconstitution, -20 °C as supplied

    数据库链接
    Accession

    参考图片

    IHC shows positive staining in paraffin-embedded human liver.

    Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.              

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human liver cancer.

    Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.              

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded mouse liver.

    Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.              

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded mouse stomach.

    Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.              

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded rat liver.

    Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.              

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    WB result of LDL-receptor Rabbit mAb                

    Primary antibody: LDL-receptor Rabbit mAb at 1/500 dilution
    Lane 1: LNCaP whole cell lysate 20 µg
    Lane 2: HEK293 whole cell lysate 20 µg
    Lane 3: PC-3 whole cell lysate 20 µg
    Lane 4: HepG2 whole cell lysate 20 µg
    Lane 5: Hela whole cell lysate 20 µg
    Low expression control: HEK293 whole cell lysate    

     low expression control: LNCaP whole cell lysate      

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 140 kDa
    Observed MW: 140 kDa
    Exposure time: 120s

    WB result of LDL-receptor Rabbit mAb                

    Primary antibody: LDL-receptor Rabbit mAb at 1/500 dilution
    Lane 1: RAW264.7 whole cell lysate 20 µg
    Lane 2: rat liver whole cell lysate 20 µg

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 140 kDa
    Observed MW: 140 kDa
    Exposure time: 180s

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    货号:
    S0B2054-10ul
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