Human Lineage Cell Depletion Set - DM

品牌： BD Pharmingen

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参数

反应种属：

Human

实验应用：

Cell separation (Routinely Tested)

产品介绍

产品信息

耦联标记

Magnetic Bead

抗原名称

Lineage

简单描述

The BD IMag™ Human Lineage Cell Depletion Set - DM is used for the depletion of mature hematopoietic cells such as T cells, B cells, NK cells, platelets, monocytes, granulocytes, and erythroid cells from peripheral blood mononuclear cells (PBMC), bone marrow and cord blood samples. This allows for rare cell populations such as stem cell, progenitor cells and dendritic cells to be enriched. The Human Lineage Cell Depletion Cocktail contains biotinylated monoclonal antibodies that recognize antigens expressed on T cells, B cells, NK cells, monocytes, granulocytes, platelets, and erythroid cells. The BD IMag™ Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag™ Human Lineage Cell Depletion Set - DM can be used as a primary enrichment tool or a pre-flow sorting tool for your rare cell population enrichment. As a pre-flow cytometric sorting tool, it can reduce sorting time dramatically in addition to increasing the recovery of the cells of interest. This Human Lineage Cell Depletion Set has been optimized for use with the BD IMag™ Cell Separation Magnet, and it contains sufficient reagents to label 1 x 10^9 peripheral blood mononuclear cells (PBMC). The Human Lineage Depletion Cocktail is comprised of the following biotinylated monoclonal antibodies: Biotin Mouse Anti-human CD3, clone UCHT1 Biotin Mouse Anti-human CD14, clone M5E2 Biotin Mouse Anti-human CD16, clone 3G8 Biotin Mouse Anti-human CD19, clone HIB19 Biotin Mouse Anti-human CD41a, clone HIP8 Biotin Mouse Anti-human CD56, clone B159 Biotin Mouse Anti-human Glycophorin A, clone GA-R2

BD化合物表

描述
EntrezGene ID
Streptavidin Particles Plus – DM
N/A
Human Lineage Cell Depletion Cocktail
N/A

应用

实验应用

Cell separation (Routinely Tested)

反应种属

Human

参考图片

Depletion of lineage cells from human peripheral blood. PBMC were labeled with the BD IMag™ Human Lineage Cell Depletion Set - DM (Cat. No. 560030) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a human lineage cocktail consisting of PE Mouse Anti-Human CD3 (Cat. No. 555333), CD14 (Cat. No. 555398), CD16 (Cat. No. 555407), CD19 (Cat. No. 555413), CD56 (Cat. No. 555516), CD235a (Cat. No. 555570) to detect lineage cells, and an APC Mouse Anti-Human CD45 (Cat. No. 555483) to detect non-lineage cells. Dead cells were excluded by staining with 7-Amino-actinomycin D (7-AAD) (Cat. No. 559925). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of non-lineage cells is indicated in each panel. Left panel shows the unseparated PBMC while the middle and right panels show the twice-enriched fraction after three 6-minute magnetic separations with an additional 10 minute separation (middle) and the positive fraction (right).

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货号：

560030

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