1/2
品牌： BD Pharmingen
下载产品说明书
用小程序，查商品更便捷
收藏
对比
咨询反应种属：
Mouse (QC Testing)
Mouse (QC Testing)
来源宿主：
Rat LEW, also known as Lewis IgG2a, κ
Rat LEW, also known as Lewis IgG2a, κ
产品介绍
产品信息
耦联标记
PE
抗原名称
Ly-6G
宿主
Rat LEW, also known as Lewis IgG2a, κ
免疫原
Ly-6G-transfected EL4J Cell Line
简单描述
The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
商品描述
The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
同种型
Rat LEW, also known as Lewis IgG2a, κ
克隆号
克隆 1A8 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
Ly-6G
背景
别名
Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen).
参考图片
Expression of Ly-6G on bone-marrow leukocytes. C57BL/6 bone-marrow cell suspensions were stained with either PE Rat Anti-Mouse Ly-6G (Cat. No. 551461, open histograms) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, filled histograms), in the presence of Mouse Fc Block™ purified anti-mouse CD16/CD32 (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463), and leukocyte subsets were distinguished by their light-scatter profiles. The left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
危险品化学品经营许可证(不带存储) 许可证编号：沪(杨)应急管危经许[2022]202944(QY) 
营业执照(三证合一)