LysoTracker ® Green DND-26

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实验应用：

IF-IC

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Product Usage Information

Each vial of LysoTracker® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX!To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.

组成成分

二甲基亚砜95-<100%，绿色有机染料1-2%，水<5%

应用

实验应用

IF-IC

背景

LysoTracker® Green DND-26 is a cell permeable green dye that stains acidic compartments (lysosomes) in live cells.

制备和贮存

保存方式

Store at -20°C dessicated and protected from light. This reagent is stable for 12 months. Seal vial tightly after use to refreeze. Avoid excessive freeze-thaw cycles.

参考图片

Confocal immunofluorescent analysis of live HeLa cells, untreated (left) or chloroquine-treated (50 μM, 4 hr; right), using LysoTracker® Green DND-26 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析活的HeLa细胞，未处理（左图）或用氯喹处理(50 μM, 4小时;右图)，使用LysoTracker® Green DND-26（绿色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

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货号：

8783S

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询价

500ul

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