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Phospho-MAP2 (Ser136) Antibody

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Phospho-MAP2 (Ser136) Antibody

品牌： CST

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参数

分子量：

75, 82, 280

反应种属：

Human,Rat,Monkey,

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产品介绍

产品信息

抗原名称

MAP2

来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser136 of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.

宿主

Rabbit

简单描述

Polyclonal Antibody for studying MAP2 (Ser136) phosphate. Cited in 2 publications. Validated for Western Blotting, Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Phospho-MAP2 (Ser136) Antibody (CST #4541) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Frozen)	1:100

分子量

75, 82, 280

内毒素水平

小鼠

研究领域

神经科学

应用

反应种属

Human,Rat,Monkey,

预测反应种属

Mouse

目标/特异性

Specificity/Sensitivity

Phospho-MAP2 (Ser136) Antibody detects endogenous levels of all isoforms of MAP2 only when phosphorylated at serine 136. This antibody does not cross-react with phosphorylated tau. Non-specific labeling of mouse pancreas and colon may be observed by immunofluorescence.

Species Reactivity:

Human, Rat, Monkey

敏感性

Endogenous

背景

Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3). 1.Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168. 2.Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130. 3.Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

研究领域

神经科学

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

4133

UniProt ID

P11137

参考图片

Western blot analysis of extracts from COS cells, untransfected (lane 1) or MAP2C-transfected (lanes 2,3), using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of the antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)

Western blot分析COS细胞，未转染（列1）或转染MAP2C（列2、3），使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗（上图）或MAP2 Antibody 兔多抗 #4542（下图）。列3中，裂解液经过λ磷酸酶处理，以显示抗体的磷酸化特异性。（人HAP2C载体由 Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY的Dr. B. Shafit-Zagardo赠送）

Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower).

Western blot分析PC12细胞提取物，未处理或nocodazole处理，使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗（上图）或MAP2 Antibody 兔多抗 #4542（下图）。

Confocal immunofluorescent analysis of rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-MAP2 (Ser136) Antibody (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析大鼠大脑，未经处理（左图）或经过λ磷酸酶处理（右图），使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗（绿色）和β3-Tubulin (TU-20) Mouse mAb 鼠单抗 #4466（红色）。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。

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货号：

4541S

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询价

100ul

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