首页抗体一抗一抗

MAP2 Antibody

1/2

MAP2 Antibody

品牌： CST

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

分子量：

75，82，280

反应种属：

Human,Mouse,Rat,Monkey,

展开

产品介绍

产品信息

抗原名称

MAP2

来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.

宿主

Rabbit

简单描述

Polyclonal Antibody for studying MAP2. Cited in 164 publications. Validated for WB, IF, IF. Highly specific and rigorously validated in-house, MAP2 Antibody (CST #4542) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Frozen)	1:50
Immunofluorescence (Immunocytochemistry)	1:400 - 1:1600

分子量

75，82，280

研究领域

神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

MAP2 Antibody detects endogenous levels of all isoforms of MAP2 total protein. This antibody does not cross-react with tau. Non-specific labeling of mouse pancreas, colon, small intestine, and liver may be observed by immunofluorescence.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

微管结合蛋白 2 (MAP2) 是一种神经元磷蛋白，可调节微管的结构和稳定性、神经元形态形成、细胞骨架动力学以及轴突和树突细胞中的细胞器转运 (1)。神经元中有多种 MAP2 同工型表达，包括高分子量 MAP2A 和 MAP2B（280 和 270 kda）和低分子量 MAP2C 和 MAP2D（70 和 75 kda）。MAP2 磷酸化可调节与细胞骨架的结合，并且受发育调控。GSK-3 和 p44/42 MAP 激酶在 Ser136、Thr1620 和 Thr1623 位点磷酸化 MAP2 (2,3)。GSK-3 在 Thr1620/1623 的磷酸化会抑制 MAP2 与微管的结合以及微管稳定性 (3)。 Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

研究领域

神经科学

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

4133

UniProt ID

P11137

参考图片

Western blot analysis of extracts from COS cells, untransfected or MAP2C transfected, using MAP2 Antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)

Western blot分析未转染或转染MAP2C的COS细胞，使用的抗体是MAP2 Antibody 兔多抗。（人HAP2C载体由Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY的Dr. B. Shafit-Zagardo赠送）

Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using MAP2 Antibody.

Western blot分析未处理或nocodazole处理的PC12细胞，使用的抗体是MAP2 Antibody 兔多抗。

Confocal microscopic images of PC-12 cells showing cytoplasmic stain with MAP2 Antibody (A, red) compared to an isotype control (B). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

PC-12细胞的共聚焦显微图像显示出MAP2 Antibody 兔多抗对胞质染色（A图，红色），与同型对照比对（B图）。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。

Confocal immunofluorescent analysis of rat cerebellum, hippocampus and spinal cord (as indicated) using MAP2 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析大鼠小脑、海马和脊髓，使用的抗体是MAP2 Antibody 兔多抗（绿色）。蓝色伪彩=DRAQ5® #4084 (荧光DNA染料)。

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

4542S

一键复制

询价

供应商现货

100ul

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价