首页抗体一抗一抗

MDM2 (D1V2Z) Rabbit mAb

品牌： CST

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

分子量：

反应种属：

Human

展开

产品介绍

产品信息

抗原名称

MDM2

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val280 of human MDM2 protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying MDM2. Cited in 105 publications. Validated for WB, IP, IF. Highly specific and rigorously validated in-house, MDM2 (D1V2Z) Rabbit Monoclonal Antibody (CST #86934) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunofluorescence (Immunocytochemistry)	1:200 - 1:800

分子量

研究领域

癌症,细胞生物学,神经科学

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

MDM2 (D1V2Z) Rabbit mAb recognizes endogenous levels of total MDM2 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, G, and 11. This antibody may detect isoform A1.

Species Reactivity:

Human

敏感性

Endogenous

背景

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3). 1.Haupt, Y. et al. (1997) Nature 387, 296-299. 2.Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. 3.Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. 4.Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

研究领域

癌症,细胞生物学,神经科学

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

4193

UniProt ID

Q00987

参考图片

Western blot analysis of SJSA-1, U-2 OS, and Saos-2 cells, untreated (-) or treated with Nutlin 3a (10 μM, 24 hr; +), using MDM2 (D1V2Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower).

Confocal immunofluorescent analysis of U-2 OS cells, untreated (left) or treated with Nutlin 3a (10 μM, 24 hr; right), using MDM2 (D1V2Z) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

86934S

一键复制

询价

供应商现货

20μl

100ul

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价