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Features
- Custom-designed target site blockers for specific inhibition of miRNA targets
- Sophisticated design and superior high affinity, regardless of target sequence
- Unmatched high efficacy in vitro and in vivo
- Unrivaled performance and high protein expression due to lack of RNase H-dependent mRNA degradation
- Efficient at very low concentrations, outcompeting miRNAs for their target sites
- Superior biological stability for long-lasting antisense activity
Product Details
miRCURY LNA miRNA Power Target Site Blockers are antisense oligonucleotides that bind to the miRNA target site of an mRNA, preventing miRNAs from gaining access to that site. This enables you to study the effects of an miRNA on a single target. miRCURY LNA miRNA Power Target Site Blockers are efficient at very low concentrations, and due to their high affinity from LNA enhancement, the target site blockers outcompete miRNAs for their target sites.
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Performance
Unravel miRNA function with target site blockers
miRNAs typically regulate gene expression of multiple targets, and inhibition of an miRNA will result in derepression of all of these targets. Therefore, a phenotype observed upon miRNA inhibition is a composite result of derepression of several targets (see figure miRNA Inhibitors vs. target site blockers). However, deregulation of a few of these targets will often contribute significantly to the phenotype. Identifying these targets is important to understanding the function of the miRNA.miRCURY LNA miRNA Power Target Site Blockers can be used to:
- Determine which pathway is involved in a phenotype observed upon miRNA inhibition
- Determine which miRNA/mRNA interactions are most important in a pathway containing several predicted targets
Examples of target site blocker applications are described in figures Examples of target site blocker applications – pathways X and Y and Examples of target site blocker applications – pathway Z.
The figure The CAV1 target site blocker phenocopies the miR-199a-5p inhibitor in many important aspects shows an example of using a target site blocker to demonstrate how miR-199a-5p functions as a key effector of TGFβ signaling in lung fibroblasts.
See figures
Principle
What are target site blockers?
miRCURY LNA miRNA Target Site Blockers are LNA-enhanced antisense oligonucleotides that bind to the miRNA target site of an mRNA, thereby preventing miRNAs from gaining access to that site. This allows researchers to study the effects of an miRNA on a single target. In contrast, the phenotype observed when inhibiting an miRNA reflects the combined effects of that miRNA on all targets.
LNA-enhanced target site blockers
The incorporation of LNA into the miRCURY LNA miRNA Power Target Site Blocker means that they will compete more effectively with the miRNA/RISC complex for the miRNA target site. In addition, LNA distribution throughout the LNA/DNA mixmer ensures that the antisense oligonucleotide does not catalyze RNase H-dependent degradation of the mRNA. As a result, the TSB will cause increased expression of the protein encoded by the targeted mRNA by preventing miRNA-mediated translational attenuation (see figure LNA-enhanced target site blockers compete effectively with RISC for miRNA binding site).
See figures
Procedure
Applications
- Determining which pathway is involved in a phenotype observed upon miRNA inhibition
- Determining which miRNA/mRNA interactions are most important in a pathway containing several predicted targets
危险品化学品经营许可证(不带存储) 许可证编号：沪(杨)应急管危经许[2022]202944(QY) 
营业执照(三证合一)