miRCURY LNA SYBR Green PCR Kit (600)

• Optimized for use with miRCURY LNA miRNA PCR Assays and Panels
• Sensitive and specific detection and quantification of miRNA
• Includes optional ROX dye for optional normalization of fluorescence

The miRCURY LNA SYBR Green PCR Kit contains a high-performance PCR master mix specifically designed for use with the miRCURY LNA miRNA PCR System. The miRCURY SYBR Green PCR Kit also features a built-in control for visual identification of correct template addition, as well as Q-Bond, an additive in the PCR buffer that enables short cycling steps without loss of PCR sensitivity and efficiency. The kit has been optimized for use with any real-time cycler. ROX Reference Dye is provided in a separate tube and can be added if your cycler requires ROX as a passive reference dye.

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Unmatched sensitivity

Universal reverse transcription combined with  LNA-enhanced and T_m-normalized primers enables accurate and reliable quantification of individual miRNAs from as little as 1 pg total RNA (see figure  Accurate quantitation from down to 1 pg total RNA starting material). With exceptional sensitivity and extremely low background, you can accurately quantify very low levels of miRNA without pre-amplification. This makes the miRCURY LNA miRNA PCR System suitable for all types of samples, especially those with low RNA content, such as serum, plasma and other biofluids.

High-performance reagents for quantitative, real-time RT-PCR

• Mature miRNA quantification and profiling
• Mature miRNA and precursor miRNA detection in parallel
• snoRNA RNA detection