miRNeasy Serum/Plasma Advanced Kit (50)

• Phenol-free protocol – no need for phase separation or working under the hood
• MinElute columns allow for small elution volumes (approx. 20 µl)
• Consistent, pure RNA for all downstream applications

The miRNeasy Serum/Plasma Advanced Kit enables the isolation of total RNA, including miRNA, from a minimum of 200 µl of sample. The phenol-free protocol allows all steps to be performed on the bench and removes the need for phase separation, making the kit suitable for automation, e.g., on the QIAcube Connect. The kit includes UCP columns that go through a rigorous ultraclean production process to ensure highly pure RNA eluates.

The miRNeasy Serum/Plasma Advanced Kit provides a phenol-free alternative to the miRNeasy Serum/Plasma Kit with similar RNA yields and quality (see figure  High recovery of miRNA without the need for phenol). The miRNeasy Serum/Plasma Advanced Kit efficiently isolates total RNA, including miRNA, from serum and plasma samples starting from a minimum input amount of 200 µl. The phenol-free protocol uses easy-to-automate MinElute spin column technology without the need for a vacuum pump (see figure  miRNeasy Serum/Plasma Advanced Kit procedure). Extracellular vesicles are lysed to allow analysis of RNA in and outside of vesicles (see figure  Detection of miRNAs preferentially found inside and outside of extracellular vesicles). Unlike kits from other suppliers, the miRNeasy Serum/Plasma Advanced Kit combines ease-of-use and a phenol-free protocol without any compromise on RNA quality or yield (see figure  Competitive yields and performance).

• Biomarker discovery
• Expression profiling
• Next-generation sequencing
• Quantitative, real-time RT-PCR
• Microarray analysis