MitoTracker ® Green FM

品牌： CST

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产品介绍

产品信息

耦联标记

Green

简单描述

Cellular Dyes for studying Mito Tracker in the research area.

商品描述

Product Usage Information

Each vial of MitoTracker® Green FM contains 50 µg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 74.4 µl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into normal growth media at a concentration between 100-400 nM and incubating between 15-30 min at 37ºC. After incubation, the cells must be imaged live; DO NOT FIX!To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.

背景

MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.

制备和贮存

保存方式

Store lyophilized solid at -20°C dessicated and protected from light. In lyophilized form, this reagent is stable for 12 months. Once reconstituted in DMSO, the solution should be stored at -20°C, protected from light, and must be used within 2 weeks. Avoid freeze-thaw cycles.

参考图片

Confocal immunofluorescent analysis of HeLa cells using MitoTracker® Green FM. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞，使用MitoTracker® Green FM。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

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货号：

9074S

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询价

供应商现货

500ug

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