MitoTracker ® Red CMXRos

品牌： CST

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参数

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NONE

产品介绍

产品信息

耦联标记

Red

简单描述

Cellular Dyes for studying Mito Tracker in the research area.

商品描述

Product Usage Information

Each vial of MitoTracker® Red CMXRos contains 50 μg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 94.1 μl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into growth media at a concentration between 50-200 nM and incubation between 15-45 min at 37ºC. After incubation, fix the cells in ice-cold methanol for 15 min at -20ºC then rinse 3 times with PBS for 5 min. Proceed with immunostaining.

背景

MitoTracker® 染料是一种能渗透细胞的探针，包含与硫醇温和反应的氯甲基团，用于线粒体标记。如需标记线粒体，则细胞仅用在质膜被动扩散并聚集在活化线粒体中的 MitoTracker® 探针进行孵育。

制备和贮存

保存方式

Store lyophilized solid at -20°C dessicated and protected from light. In lyophilized form, this reagent is stable for 12 months. Once reconstituted in DMSO, the solution can be stored at -20°C, protected from light and must be used within 2 weeks. Avoid freeze-thaw cycles.

参考图片

Confocal immunofluorescent analysis of HeLa cells using MitoTracker® Red CMXRos (red) and Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞，使用MitoTracker® Red CMXRos（红色）和Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617（绿色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

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货号：

9082S

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询价

供应商现货

500ug

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